a nonsignificant trendP = 0.054
Dissecting females 10 min after starting a second mating, we found a nonsignificant trend for slowed entry of second male sperm in previously Dad-mated females (GLM, F 1, 59 = 3.718, P = 0.054; Fig. 3 I ) and reduced displacement of first male sperm at this timepoint (first male sperm in the uterus/total first male sperm across all regions of the reproductive tract; GLM, F 1, 61 = 2.836, P = 0.097; Fig. 3 J ).