The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value ( R 2 = 0.996, slope = −3.49) and that the detection limit concentration of DNA of the primer set was 10 pg of DNA per reaction.
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Identification of <i>Heterodera glycines</i> (Tylenchida; Heteroderidae) Using qPCR.
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