The time-period over which the tissue freezes is an important consideration as damage due to ice-crystal formation may be significant, thus altering the level of cellular or sub-cellular detail that can be preserved in the sample.
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A Review of <i>ex vivo</i> Elemental Mapping Methods to Directly Image Changes in the Homeostasis of Diffusible Ions (Na<sup>+</sup>, K<sup>+</sup>, Mg<sup>2 +</sup>, Ca<sup>2 +</sup>, Cl<sup>-</sup>) Within Brain Tissue.
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