Proteomic profiling and identification of significant markers from high-grade osteosarcoma after cryotherapy and irradiation.

In order to obtain the highly significant differentially expressed proteins from our study, we filtered these proteins using the false discovery rate (FDR) of <0.1%, and the highest protein scores of >70 with a significance score of <20, and at least 2 up to ten unique peptides should be identified (Fig. 2c & Supplementary Table 1 ).

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