highly significantP < 0.05
Diet-specific microbial communities developed prior to antibiotic treatment (day 13), as indicated by nonoverlapping ellipses and highly significant analysis of similarity (ANOSIM) values ( P < 0.05; R > 0.912) for standard, high-carbohydrate, and high-fat/high-protein diets on day 13; however, the community structures of high-fat/low-protein-fed animals were not distinct ( Fig. 5a ).