Higher percentage of horse serum in culture media blocks attachment of PC12 cells.

Although there was substantial neurite outgrowth following preincubation with all percentages of horse serum (including 0%), there was more variability when using lower percentages (i.e., 0, 2.5 and 5%), and while the higher percentages (i.e., 10 and 15%) did seem to foster slightly more neurite outgrowth, there was at best marginal significance, with the comparison of 5 versus 10% horse serum preincubation coming the closest to achieving statistical significance (p = 0.078).

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