Validation of RNA-Seq Data by RT-qPCR Albeit with different degrees of overlap, the gene expression datasets obtained with RT-qPCR showed a highly significant correlation with the RNA-seq results for seven out of the nine transcripts selected for validation, i.e., VTG, MFAP4, CFHL, CFBL, APOBL, C3, and CBLN2L, ( Figure 2 ), with a R 2 Pearson correlation coefficient equal to 0.7498, and a p -value < 0.00001.
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Differential Effects of Dietary Supplementation of Krill Meal, Soybean Meal, Butyrate, and Bactocell<sup>®</sup> on the Gene Expression of Atlantic Salmon Head Kidney.
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