Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling.

Statistical significance was calculated using the two-sided Welch’s t -test for comparisons of two groups or the Tukey-Kramer test for multiple comparisons, with statistical significance set at p ≤ 0.1 (marginally significant), p ≤ 0.05, and p ≤ 0.01 (significant difference).

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Pressure exposure did not affect the expression of VE-cadherin, whereas the expression of ZO-1 increased with marginal significance under the pressure condition (Fig. 1h and Supplementary Fig. 17 ).

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