highly significantP value of < 0.001
A P value of < 0.05 was considered statistically significant, a P value of < 0.01 was considered highly significant, and a P value of < 0.001 was considered extremely significant. 3 Results 3.1 PEDV infection increases autophagy in IPEC-J2 cells The replication of PEDV in IPEC-J2 cells was measured by IFA with the monoclonal antibody (mAb) 3A6 anti-PEDV N protein as the primary antibody, and the FITC-conjugated goat anti-mouse IgG as the secondary antibody.