High-resolution cryo-EM reconstructions in the presence of substantial aberrations.

Trefoil typically is not pronounced, but for one pair of EMPIAR data sets (EMPIAR 10185 and EMPIAR 10186), and also in our data sets (not shown), it was highly significant.

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Using our formula (equation 2 ), we found that this will preserve J 0 (0.6π/4) = ∼0.94 of the original signal, a reduction in the SNR that is barely significant, and so the π/4 limit is too conservative, as was postulated by Cheng et al.

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