SM d M revealed an interesting trend: For all subcellular environments, the two negatively charged (−14, −7) species ( Fig. 4a , b ) both yielded D comparable to that of the neutral species ( Fig. 4c ), but slightly higher than that of the original mEos3.2-C1 (+2 charges) ( Figs. 2 , 3 , and 4f ).
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Single-molecule displacement mapping unveils nanoscale heterogeneities in intracellular diffusivity.
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