Logistic regression analysis showed no significant association between the used disinfectants and the antibiotic resistance of the isolates (Supplementary Table 1 ). Antibiotic susceptibility evolution An increasing trend in antibiotic resistance to ampicillin, sulfamethoxazole and trimethoprim was found between sampling t1 and t3 at the broiler pilot farm (Supplementary Fig. 1 a).
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Repeated disinfectant use in broiler houses and pig nursery units does not affect disinfectant and antibiotic susceptibility in Escherichia coli field isolates.
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