highly significantp < 0.01
p < 0.05 was considered statistically significant and highly significant when ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
p < 0.05 was considered statistically significant and highly significant when ** p < 0.01, *** p < 0.001, and **** p < 0.0001.