Optimization of isolation and transfection conditions of maize endosperm protoplasts.

1 Y = - 99.560 + 39.480 x 1 + 81.593 x 2 + 62.304 x 3 + 9.735 x 4 - 1.300 x 1 x 2 + 1.125 x 1 x 3 - 0.275 x 1 x 4 + 8.250 x 2 x 3 - 2.154 ∗ 10 14 x 2 x 4 + 1.656 x 3 x 4 - 12.393 x 1 2 - 56.173 x 2 2 - 67.927 x 3 2 - 0.873 x 4 2 The quadratic model for the ANOVA was highly significant (p < 0.0001), suggesting that the model for the regression terms was adequate, and that a higher order model would not be needed.

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