highly significantP < 0.001
All factors were highly significant ( P < 0.001), except the interaction between Stage and Trait.
All factors were highly significant ( P < 0.001), except the interaction between Stage and Trait.
Several reasons may explain the worst performance of the model with IR complexity: (1) it is likely that the sample size of the current study was not sufficient to allow us to identify effectively significant taxa; (2) the analysis used (a simple linear regression) possibly did not effectively account for the compositional nature of microbiome data; and (3) each individual taxon may have a small contribution to the overall microbial effect, while some OTU may still show moderately stronger effects.