An evolutionary approach to systematic discovery of novel deubiquitinases, applied to <i>Legionella</i>.

When comparing activity-based probes made from ubiquitin with gradually increasing side chains at position 75 (wt, G75A, and G75V), an interesting trend became visible: The wild-type versions of both lpg1148 and lpg1621 reacted equally well with the wild-type probe and the G75A probe but did not react at all with the larger G75V probe.

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