Effects of EPS-CM on lipolysis of 3T3-L1 adipocytes Since triglyceride accumulation measured by Oil Red O staining was unchanged but showed a decreasing trend on day 10 when continuously treated with EPS-CM ( Fig 2B ), we examined whether EPS-CM induced lipolysis in mature adipocytes on day 10.
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Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes.
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The amount of lipids was significantly increased by EPS-CM on day 4, and continued to show an increasing trend on day 6, but tended to decrease by day 10 ( Fig 2B ).