extremely significantp -value ≤ 0.0001
Results were analyzed by Two-way ANOVA followed by Unpaired t -test using GraphPad Prism 8.4.2 software program (GraphPad Software, San Diego, CA, USA) and the statistical difference was observed to be extremely significant at p -value ≤ 0.0001 (****) and p -value ≤ 0.001 (***). 4.