More interestingly, by using the above described purification protocol and analytical method, three of the four recombinant strains (those overexpressing kfoF ) showed a highly significant increase (more than 50%, see Table 3 ) of the molecular weight of the overproduced CPS, once again clearly demonstrating the importance of precursor supply not only for its production, but also its potential for controlling and tailoring the molecular weight of the biopolymer.
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Production and purification of higher molecular weight chondroitin by metabolically engineered Escherichia coli K4 strains.
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