highly significantP < 0.0001
Most importantly, the LC3-II/LC3-I ratio, which measures autophagy activity, showed a highly significant increase in the iridocorneal region of the aging mice ( 4 months: 1.106 ± 0.21, n = 5; 18 months: 2.178 ± 0.10, n = 4; P < 0.0001, t -test) ( Fig. 2 C).