Metastasis Suppressor NME1 Modulates Choice of Double-Strand Break Repair Pathways in Melanoma Cells by Enhancing Alternative NHEJ while Inhibiting NHEJ and HR.

WM35- and WM164-derived clones harboring the EJ5-GFP cassette for assessment of NHEJ activity (WM35 EJ5c12 and WM164 EJ5c1) both exhibited highly significant increases in %GFP-positive cells following expression of the shNME1 sequence and induction of double-stranded breaks with I-SceI ( Figure 4 B).

also in 132,142 other papers