Multi-Enzymatic Cascade One-Pot Biosynthesis of 3'-Sialyllactose Using Engineered <i>Escherichia coli</i>.

With the amount of cell extracts gradually increased, the 3′-SL content also showed an increasing trend, but there was no significant difference in 3′-SL content at the amount of 40 g/L and 48 g/L added, so the concentration of 40 g/L was used. 60 mM lactose can be completely degraded by wild-type E. coli within 4 h ( Figure 5 a).

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