<i>SIR2</i> Expression Noise Can Generate Heterogeneity in Viability but Does Not Affect Cell-to-Cell Epigenetic Silencing of Subtelomeric <i>URA3</i> in Yeast.

SIR2 expression noise can generate cell-to-cell heterogeneity in viability Surprisingly, when measuring viability to normalize the frequency of URA3 expression, we observed a highly significant difference between the Sir2 -low and Sir2 -high subpopulations in our URA3 telomeric reporter strain: while 6/10 Sir2 -low cells were viable, this proportion was just 2/10 among Sir2 -high cells ( Figure 4A and Table S2) ( P = 6.10 −3 ).

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