51 reported that the concentrations of total and available P have shown an increasing trend with increasing manure application rate.
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Long-term application of fertilizer and manures affect P fractions in Mollisol.
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The sentences
Different fertilizer treatments have significantly impacted Ca 2 -P and Ca 8 -P concentrations as deep as 0–40 cm depth ( P < 0.05), showing a decreasing trend as OM > NPK > NPK + S.
However, crop response to P fertilizer may not be significant when available P concentration in soil is higher than an optimum or agronomic threshold value 39 .