Molecular characterization of a Trichinella spiralis serine proteinase.

The suppression was dose dependent for anti-rTsSerp antibodies ( r = 0.986) and showed a decreasing trend with increasing serum dilutions ( F = 181.170, P < 0.0001) (Figure 10 F).

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When the medium was replenished with rTsSerp-supplemented medium and the IECs were cultured with the IILs for 2 h, rTsSerp obviously facilitated the larval invasion of the IECs; this facilitation was rTsSerp dose dependent ( r = 0.996) and showed a positive trend with the increase in the rTsSerp concentration ( F = 425.376, P < 0.001); however, the TRX-tag did not accelerate larval invasion (Figure 10 D, E).

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There was also a positive correlation between cytotoxicity and culture time ( r = 0.921), and cytotoxicity showed an increasing trend with prolonged culture time ( F = 18.356, P < 0.01).

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