Assessment of adipose reserves in whole zebrafish sections revealed that VDD zebrafish exhibited hyperplasia of dorsal paraosseal (dPOS) non-visceral AT (NVAT) (60.78 ± 9.09 adipocytes VDD versus 2.27 ± 1.14 adipocytes VD3 Sufficient) and pancreatic visceral AT (PVAT) (64.75 ± 15.11 adipocytes VDD versus 29.56 ± 6.03 adipocytes VD3 Sufficient), along with hypertrophy of dPOS NVAT and PVAT with an overall trend towards larger adipocytes in the VDD zebrafish (Fig. 4 ) at 6 mpf 31 .
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Vitamin D deficiency serves as a precursor to stunted growth and central adiposity in zebrafish.
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