Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain.

As shown in Figure 4D , genomic PCR of single cell clones revealed that Cas9-SB and Cas9-N57 increased the insertion efficiency from 24.1 to 36.3% and to 33.8%, respectively, although only marginal significance was observed ( P -values were 0.0495 and 0.1151 for Cas9-SB and Cas9-N57, respectively.

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