Overexpression of an ethylene-forming ACC oxidase (ACO) gene precedes the Minute Hilum seed coat phenotype in Glycine max.

The gene for an ethylene forming enzyme, ACC oxidase ( ACO ), is highly overexpressed in the mi isoline Among the many overexpressed Glyma models in all the annotation categories, Glyma.09G008400, annotated as an ethylene forming enzyme in the hormone related category, was the most highly differentially expressed gene in the mi isoline UC413 with 163 RPKMs in the EH10 sample compared to only 0.03 RPKM for the UC7 recurrent parent with a fold difference of 1.8 × 10 − 4 and highly significant padj value of 1.9 × 10 − 219 (Table 3 , Additional file 2 ).

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