Peripheral CD8<sup>+</sup> T cell characteristics associated with durable responses to immune checkpoint blockade in patients with metastatic melanoma.

This confirmed a highly significant association between day 21 large clone count and six-month outcome, with responders having on average 5.7 more large clones on day 21 (95% CI: 2.6 - 8.87, P=4.8×10 -4 , Supplementary Figure 1 ).

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