highly significantP ≤ 0.001
Effect of marker segregation distortion on map construction Single locus analysis detected 5.9% of markers with highly significant (P ≤ 0.001) distortion, and 7.3% with significant (0.001 < P < 0.05) segregation distortion, giving a total of 98 markers with distortion from the expected Mendelian segregation (Table 1 ).