The involvement of miR-6615-5p/Smad7 axis and immune imbalance in ammonia-caused inflammatory injury via NF-κB pathway in broiler kidneys.

In addition, PCC analysis showed that there was an extremely significant negative relationship (r = −0.832, P < 0.01) between miR-6615-5p expression and Smad7 mRNA expression, further confirming the target relationship between miR-6615-5p and Smad7. iNOS and NO In order to investigate if iNOS and NO were involved in NH 3 -induced inflammatory damage, iNOS activity ( Figure 3 A), iNOS mRNA ( Figure 3 B) and protein expression ( Figure 3 C), and NO content ( Figure 3 D) were measured.

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