Because the inferential value of a failure to reject the null hypothesis in frequentist statistical approaches is limited, for all RNA-seq expression datasets, we coupled our standard differential expression with a test for whether each gene could be flagged as “significantly not different.” Defining a region of practical equivalence as a change of no more than 1.5-fold in either direction, we tested the null hypothesis of at least a 1.5-fold change for each gene, using the gene-wise estimates of the SE in log 2 fold change (reported by Deseq2) and the assumption that the actual l 2 fcs are normally distributed.
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