Molecular surveillance of anti-malarial resistance Pfdhfr and Pfdhps polymorphisms in African and Southeast Asia Plasmodium falciparum imported parasites to Wuhan, China.

Although a decreasing trend was observed in the prevalence of the quadruple-mutant AG K GS from 18.5% in 2012 to 9.8% in 2016 and finally to 0% in 2019, these differences were not statistically significant (F = 0.0063, P = 0.9391).

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For IRN I-S GE AA, an increasing trend was detected during 2011–2016, and 2017–2019, respectively (Fig. 1 e).

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