Behavioral and Oxidative Stress Changes in Mice Subjected to Combinations of Multiple Stressors Relevant to Irritable Bowel Syndrome.

The Effects of Various Combinations of Stress Factors on the Parameters Evaluated in Elevated Plus Maze Regarding the number of open arms entries, a parameter for which lower values are consistent with anxiogenic–like behavior since it is based on the natural aversion of rodents for open, visible decreases were observed for all stress exposed groups as compared to control group, which were statistically significant in the MS+MF group (F (1, 18) = 6.18, p = 0.023; t(9) = 2.75, p = 0.01) and almost significant in the MS group (F (1, 18) = 3.92, p = 0.063; t(16) = 1.97, p = 0.03) ( Figure 4 a).

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Effects of Various Combinations of Stress Factors on the Parameters Evaluated in Forced Swim Test In the forced swim test, statistical analysis of the swimming time showed significantly low mobility for MS group (F (1, 18) = 6.69, p = 0.018; t(17) = 2.58, p = 0.009) and almost significantly low mobility for the other two stressed groups ( Figure 5 a).

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