16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis.

• CDC Contaminant : Present in enrichment broth only and organism consistent with skin flora and a not clearly significant organism (e.g., Coagulase negative Staphylococci [CNS], Diphtheroid, Alpha-hemolytic Streptococci, Bacillus sp. non-cereus). • 16S rRNA gene PCR Positive : ≥ 1+ band positive and organism identified by sequencing, or ≥ 1+ electrophoresis gel band positive and positive on repeat PCR. • 16S rRNA gene PCR Contaminant : 1+ electrop-horesis gel band positive only and negative on repeat PCR testing.

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