highly significantP < 0.001
Results Initial metabolite concentrations At day 0, there were highly significant differences in the concentrations of metabolites present (F 4,1074 = 262.87, P < 0.001) for all life cycle stages (F 2,1074 = 791.7, P < 0.001) with a significant interaction between metabolite and life cycle stage (F 8,1074 = 227.9, P < 0.001; Fig. 1 ).