An advanced optical clearing protocol allows label-free detection of tissue necrosis <i>via</i> multiphoton microscopy in injured whole muscle.

Statistical analyses from almost 2,500 image slices of several volume images through different areas of CTX-treated and untreated TDE-cleared muscles show a highly significant loss of fSHG signal intensity, both when normalized to AF (Figure 7 B) and bSHG (Figure 7 D) .

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