A two-way factorial ANOVA for the effects of VRN-A1 and FT-B1 on all traits revealed a highly significant effect of FT-B1 but not VRN-A1 on TSN, while both genes were found significant for the three other traits (Table 3 , Supplementary Table S5 ).
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Linkage mapping identifies a non-synonymous mutation in FLOWERING LOCUS T (FT-B1) increasing spikelet number per spike.
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