A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin.

At elevated C0–C2 concentrations, competition between sites, cooperative interactions, and steric hindrance are all likely to be significant.

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TR-F changes due to C0–C2 binding to actin are highly significant and not probe dependent All five fluorescent probes we tested reported a reduction in lifetime upon binding ( Fig.

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