Conversely, preincubation with DTT is the unique condition boosting the activity of cleaved toxin ( Figure 6 B), as exemplified by the extremely significant difference (~140 fold) in the IC 50 obtained with trypsin-treated Stx2a in the presence of protein G treated-serum under non-reducing and reducing conditions (y-intercept difference, p = 0.0007).
← all excerpts
Method for the Detection of the Cleaved Form of Shiga Toxin 2a Added to Normal Human Serum.
1
—
—