The highly significant differences between cord blood and maternal peripheral blood ( p < 0.0001) were also preserved upon subdivision according to maternal allergy status (see Figure 2 c,d and Supplementary Figure S4b for total Treg, iTreg and nTreg, respectively) as well as according to the sex of the newborns (see Figure 2 e,f and Supplementary Figure S4c for total Treg, iTreg and nTreg, respectively). 3.3.
Although the difference was not significant, a slight trend towards lower expression of regulatory cytokines in Treg isolated from the cord blood of children of allergic mothers is discernible. 3.8.
In our coculture-based assay, we did not see any significant difference between stimulated conventional T cells cultured with or without Treg for any studied parameters, although a nonsignificant trend toward lower IL-10 secretion was discernible in samples isolated from the cord blood of children of allergic mothers, consistent with our hypothesis of more robust regulatory cells in the low-risk group.