extremely significantp < 0.001
However, in the acetic-acid induced writhing inhibition test, EaF-SF at the doses of 200 and 400 mg/kg revealed an extremely significant ( p < 0.001) reduction in the number of writhing 34.75 ± 0.85 and 24.75 ± 0.62, with 24.86% and 46.49% writhing inhibition, respectively compared to the negative control (46.25 ± 1.54).