Characterization of gene expression revealed an expected trend for both encapsulated and control spheroids—expression of pluripotency genes ( OCT4, SOX2, NANOG ) was high with cells in pluripotency (mTeSR) media at days 1 and 3 but then decreased after spheroids were transferred into endodermal differentiation media during subsequent 3 days (Fig. 6 B,C).
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Core-shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor.
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