Materials and methods Sequence retrieval For analyzing the genome of the novel coronavirus we utilized the completely sequenced Wuhan-based SARS-CoV-2 genome (accession ID— NC_045512.2 ) available on NCBI, while, for proteomic analysis, we deployed the SARS-CoV protein sequence (UniProtID: P0C6X7 /RNA-dependent RNA polymerase (RdRp) accession ID: YP009725307.1). Sequence similarity, alignment and phylogenetic analysis For identifying sequences that are highly similar to the query sequence of RdRp, we employed the Basic Local Alignment Search Tool (BLAST) [ 1 , 6 ], and essentially significant application found in NCBI.
← all excerpts
In silico approach to understand the epigenetic mechanism of SARS-CoV-2 and its impact on the environment.
1
—
—