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Genetic changes are introduced by repeated exposure of Salmonella spiked in low water activity and high fat matrix to heat.

Sci Rep · 2021 · PMC8046991 · PMID 33854082

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an increasing trendno p-value reported
The number of differences accrued with every additional heat treatment cycle performed thus showing an increasing trend per cycle.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.