A non-destructive sugar-feeding assay for parasite detection and estimating the extrinsic incubation period of Plasmodium falciparum in individual mosquito vectors.

As expected, there was a highly significant positive relationship between time post-bloodmeal and the proportion of mosquitoes with ruptured oocysts ( LRT X 2 1 = 147, P < 0.001, Fig. 1 a).

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