Interestingly, we observed that the co-expression of sgRNA and Cas9 reduced the number of GFP-positive cells early on at 2 dpf and induced a highly significant decrease from 3 dpf onwards, in comparison to Cas9 expression alone that had a more modest initial impact and reduced the number of cells only from 3 dpf onward ( Figure 2 D).
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<i>foxm1</i> Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis.
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