Despite a slight trend in favor of protocol 1, no statistically significant differences were observed between protocols at any time point analyzed throughout EB development regarding the frequency of hemogenic endothelial (HE) precursors; CD43+ CD45−, CD45+, and CD45 + CD34 + hematopoietic derivatives; or the output of clonogenic progenitors.
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A Benchmark Side-by-Side Comparison of Two Well-Established Protocols for <i>in vitro</i> Hematopoietic Differentiation From Human Pluripotent Stem Cells.
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