highly significantP < 0.05
There was a statistically significant difference ( P < 0.01 implies highly significant difference, and P < 0.05 implies significant difference) seen for the values between the time intervals baseline–1 min, baseline–2.5 min, and 1–2.5 min, indicating that there were a lesser number of colony forming units at 1 and 2.5 min when compared to baseline and at 2.5 min when compared 1 min.