RNAi#1 caused a weak trend toward RU-dependent sleep loss that did not reach significance when crossed with Actin-GS> Dicer2, likely because of weaker knockdown (data not shown); note also that RNAi#2 is predicted to have higher specificity ( Figure 2—figure supplement 2D ).
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Short and long sleeping mutants reveal links between sleep and macroautophagy.
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